The Olive Leaf Extract Induced Phenoloxidase Activity Changes in Galleria mellonella Hemolymph

Since the fruits and leaves of Olive (Olea europea) tree, which grows naturally in Mediterranean countries, are thought to be beneficial for many diseases, it is widely used folk medicine these countries. leaf extract has been treatment diseases such as malaria, high fever, heart cancer. The greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) frequently invertebrate immunity studies due its similarity mammalian natural immune system. Melanization occurs when quinone precursors converted melanin inactive profenoloxidase phenoloxidase enzyme. In this study, activity G. hemolymph larvae injected with olive different doses was determined by ELISA microplate reader at 492 nm absorbance. findings obtained from study showed that increased a dose 0.001 mg mL-1. Lower mL-1 did not cause changes compared untreated Dimethyl Sulfoxide (DMSO) groups. highest our 0.01 survive 24 hours over those doses. results show supports humoral responses low